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On-line process monitoring and chemometric modeling with 2D fluorescence spectra obtained in recombinant E. coli fermentations

Paper ID Volume ID Publish Year Pages File Format Full-Text
35868 45111 2007 11 PDF Available
Title
On-line process monitoring and chemometric modeling with 2D fluorescence spectra obtained in recombinant E. coli fermentations
Abstract

2D spectrofluorometry produces a large volume of spectral data during fermentation processes with recombinant E. coli, which can be analyzed using chemometric methods such as principal component analysis (PCA), principal component regression (PCR) and partial least square regression (PLS). An analysis of the spectral data by PCA results in scores and loadings that are not only visualized in the score-loading plots but are also used to monitor the fermentation processes on-line. The score plots provided useful qualitative information on four fermentation processes for the production of extracellular 5-aminolevulinic acid (ALA). Two chemometric models (PCR and PLS) were used to examine the correlation between the 2D fluorescence spectra and a few parameters of the fermentation processes. The results showed that PLS had slightly better calibration and prediction performance than PCR.

Keywords
Chemometric modeling; Fermentation with E. coli; PCA; PCR; PLS; On-line monitoring; 2D fluorescence spectra
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On-line process monitoring and chemometric modeling with 2D fluorescence spectra obtained in recombinant E. coli fermentations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 7, July 2007, Pages 1124–1134
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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