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Regeneration of denatured polyphenol oxidase in Toona sinensis (A.Juss.) Roam

Paper ID Volume ID Publish Year Pages File Format Full-Text
35873 45111 2007 5 PDF Available
Title
Regeneration of denatured polyphenol oxidase in Toona sinensis (A.Juss.) Roam
Abstract

The regeneration of polyphenol oxidase (PPO) from leaves of Toona sinensis (A.Juss.) Roam (TS), denatured by boiling with 0.4% (w/v) sodium dodecyl sulfate (SDS) and 1% (v/v) β-mercaptoethanol, and was studied with SDS-polyacrylamide gel electrophoresis (PAGE) and the gel-activity-stained assay. The optimal regenerating conditions for the denatured TS-PPO were as follows: extracting with buffer of 150 mM Na-Pi, pH 7.2; regenerating with buffer of 50 mM Tris–HCl, pH 7.2, 2% Triton X-100 for 45 min. In addition, the PPO regeneration was promoted by the addition of Cu2+ in the recovery buffer. The regenerating activity of the denatured-PPO was reduced in samples extracted with buffers containing polyvinylpyrrolidone (PVP), polyvinylpolypyrrolidone (PVPP) or Triton X-100.

Keywords
Toona sinensis; Polyphenol oxidase; Denaturing; Renaturing; SDS-PAGE; Gel-activity-stain assay
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Regeneration of denatured polyphenol oxidase in Toona sinensis (A.Juss.) Roam
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 7, July 2007, Pages 1155–1159
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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