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Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system

Paper ID Volume ID Publish Year Pages File Format Full-Text
3589 177 2012 5 PDF Available
Title
Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system
Abstract

A recombinant Pichia pastoris displaying Rhizomucor miehei Lipase (RML) on the cell surface was shown to be an efficient biocatalyst in biodiesel production from soybean oil. Various reaction parameters affecting the transesterification catalyzed by RML-displaying P. pastoris whole cells were studied. The optimal reaction conditions were: temperature 55 °C, water activity 0.66, the ratio of isooctane to oil 2:1 (v/v) and the whole-cell biocatalyst 40% (w/w, soybean oil). To reduce the inactivation of displaying lipases by methanol, each 1.5 molar equivalent of methanol (mol/mol, soybean oil) were added in a three-step procedure at the reaction time of 0 h, 24 h and 48 h, respectively. Under these optimized conditions, a methyl esters (ME) yield reached 83.14% after 72 h, which significantly enhanced the ME yield catalyzed by the displaying lipase reported previously. The RML-displaying P. pastoris whole-cell biocatalyst exhibited quite a good operational stability with more than 80.46% of its original activity remained even after 10 batches reaction as well as storage stability. Thus, it showed promising potential in biodiesel production.

Keywords
Biodiesel; Pichia pastoris; Rhizomucor miehei Lipase; Three-step methanolysis; Whole-cell biocatalyst; Yeast-displaying
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Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 63, 15 April 2012, Pages 10–14
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us