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Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels

Paper ID Volume ID Publish Year Pages File Format Full-Text
35903 45113 2007 6 PDF Available
Title
Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels
Abstract

The crystallization of lysozyme was monitored in 20-μl sitting-drop vapor diffusion experiments and a quantitative phase diagram was obtained. Then, batch crystallization of lysozyme in shaked 200-μl microtiter plates was investigated. It was observed that with rising agitation rates, the area of the nucleation zone was significantly reduced. Further batch crystallization experiments were performed (i) in 0.2–2-ml Eppendorf tubes in a laboratory rotator, (ii) in 5-ml unbaffled shake flasks, and (iii) in 4-ml stirred baffled and unbaffled vessels. The crystal area density distributions of the stirred vessels were clearly more narrow compared to the rotated Eppendorf tubes. The crystal area density distributions of the shake flasks were significantly wider. The use of the biocompatible, water-soluble ionic liquid ethanolammonium formate as a crystallization additive in unbaffled stirred vessels resulted in larger, sturdy crystals and reduced formation of crystal aggregates. The experiments indicate that ml-scale batch crystallization of lysozyme in stirred vessels can be performed fast, up-scaleable, reasonably reproducible, and precipitation can be avoided reliably.

Keywords
Agitated ml-scale vessels; Batch crystallization; Ethanolammonium formate; Ionic liquid; Lysozyme; Vapor diffusion experiments
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Crystallization of lysozyme: From vapor diffusion experiments to batch crystallization in agitated ml-scale vessels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 12, December 2007, Pages 1649–1654
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us