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Sucrose conversion into palatinose with immobilized Serratia plymuthica cells in a hollow-fibre bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
35904 45113 2007 5 PDF Available
Title
Sucrose conversion into palatinose with immobilized Serratia plymuthica cells in a hollow-fibre bioreactor
Abstract

In recent decades, the production of palatinose has aroused great interest since this structural isomer of sucrose has a promising potential. Using immobilized in a hollow-fibre membrane reactor Serratia plymuthica cells, a complete conversion of concentrated sucrose solutions into palatinose was achieved. Under typical process conditions, the specific productivity of the membrane reactor was 16.8 g m−2 h−1 (flow rate 1.3 cm3 min−1 and substrate concentration 40%) in continuous mode of action. The activity of the biocatalyst (productivity of the system) decreased slowly with the increase of operation time until the 15th day and remained almost constant to the end of the experiment. The loss of activity was 11% after 90 days of continuous operation. Conversion rate of over 90% was reached for 36–48 h for all concentrations (40–60%) of the substrate solution in cycle mode of action of the bioreactor. The best productivity (18.1 g palatinose m−2 biocatalytic membrane) for the set period was observed during the recirculation of a 60% sucrose solution. The culture displayed a very good stability under the conditions of critical osmotic stress during the experiments. A microbiological analysis of the end product showed that the produced palatinose syrup measures up to the standards for products of this kind and can be used as an additive to different food products and functional foods.

Keywords
Palatinose; Serratia plymuthica; Immobilized cells; Hollow-fibre reactor
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Sucrose conversion into palatinose with immobilized Serratia plymuthica cells in a hollow-fibre bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 12, December 2007, Pages 1655–1659
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us