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Biochemical properties and thermal behavior of pectate lyase produced by Pectobacterium carotovorum subsp. carotovorum BR1 with industrial potentials

Paper ID Volume ID Publish Year Pages File Format Full-Text
3591 177 2012 9 PDF Available
Title
Biochemical properties and thermal behavior of pectate lyase produced by Pectobacterium carotovorum subsp. carotovorum BR1 with industrial potentials
Abstract

An extracellular pectate lyase (PL, EC 4.2.2.2) produced by Pectobacterium carotovorum subsp. carotovorum BR1 was purified and characterized with respect to its biochemical properties including the thermodynamic parameters of substrates hydrolysis and thermal behavior. The PL showed higher affinity for polygalacturonic acid (Km, 0.4 g l−1) as compared to 70% methylated pectin (Km, 0.77 g l−1). Nonlinear thermal inactivation curves of PL at 50 and 60 °C, fitted to a three-fraction first-order kinetic model. Furthermore, the thermodynamic parameters of thermal deactivation of purified PL viz. ΔH*, ΔS*, Ed and ΔG* were determined over pH range 6–10 and in presence of different cations. Alkaline pH and Ca2+ ions individually influenced the thermostability of PL at elevated temperatures 60 and 70 °C, based on the thermodynamic parameters. The studies suggested that this alkaline PL can be considered as potential candidate for various applications like pectic waste management, degumming of fibers, food, paper and textile industries.

► Kinetics and thermodynamics parameters of the pectate lyase (PL) were determined. ► Thermal inactivation curves of PL fitted three-fraction first-order kinetic model. ► Alkaline pH and Ca2+ ions favor the thermostability of purified PL at 60 and 70 °C. ► Thermoactive PL has an alkaline pH optimum, low Ea and broad substrate specificity. ► PL could be utilized efficiently as a novel source for industrial applications.

Keywords
PL, pectate lyase; PG, polygalacturonase; PNL, pectin lyase; Pcc, Pectobacterium carotovorum subsp. carotovorum; Ecc, Erwinia carotovora subsp. carotovora; Eca, E. carotovora subsp. atroseptica; Ech, E. chrysanthemi; w/v, weight per volume; ΔH#, enthalpy
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Biochemical properties and thermal behavior of pectate lyase produced by Pectobacterium carotovorum subsp. carotovorum BR1 with industrial potentials
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 63, 15 April 2012, Pages 22–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us