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Production of chitosan oligosaccharides using chitosanase immobilized on amylose-coated magnetic nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
35917 45114 2008 8 PDF Available
Title
Production of chitosan oligosaccharides using chitosanase immobilized on amylose-coated magnetic nanoparticles
Abstract

We developed chitosanase immobilized on amylose-coated magnetic nanoparticles and utilized it to produce chitosan oligosaccharides. The magnetic nanoparticles used consisted of magnetite (Fe3O4) and their surface was coated with amylose. Hydroxyl groups of amylose deposited on the nanoparticles were activated chemically and then used to immobilize the chitosanase via multipoint covalent bonds. Compared to the conventional physical-adsorption method, 1.4–2.0 times the amount of chitosanase was immobilized via multipoint covalent bonding under the same experimental conditions. The immobilized chitosanase was used to produce pentamers and hexamers of chitosan oligosaccharides, which possess beneficial biological activities. In batch hydrolysis of chitosan using the immobilized chitosanase, a high yield of target oligosaccharides (40% of the used chitosan, based on weight) was successfully obtained. The immobilized chitosanase could be recovered and reused repeatedly.

Keywords
Chitosan oligosaccharide; Enzyme immobilization; Magnetic nanoparticle; Chitosanase; Multipoint attachment; Magnetic separation
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Production of chitosan oligosaccharides using chitosanase immobilized on amylose-coated magnetic nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 1, January 2008, Pages 62–69
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us