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Candida rugosa lipase immobilized in Triton-X100 microemulsion based organogels (MBGs) for ester synthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
35918 45114 2008 6 PDF Available
Title
Candida rugosa lipase immobilized in Triton-X100 microemulsion based organogels (MBGs) for ester synthesis
Abstract

The esterification ability of the lipase VII from Candida rugosa was studied in the quaternary water-in-oil microemulsion Triton-X100/water/1-hexanol/n-hexane based organogels. The gelatin concentration exerted a high influence on both the MBG formation and the esterification rate exhibiting highest esterification at 6% (w/v). Nature of cosurfactants and oil exploited in making of the MBGs also affected the lipase-catalyzed esterification, showing highest esterification using formulations derived from 1-hexanol and n-hexane. Ethyl butyrate formation by C. rugosa lipase increased while increasing mole ratio of water/surfactant (W0) at a particular mole ratio of cosurfactant/surfactant (P0). Recycling the immobilized enzyme was shown to be feasible, demonstrating that lipase retains its activity for several cycles. Immobilization did not change the optimum temperature for both free and immobilized lipase (37 °C), however, the half-life of the enzyme increased from 14.9 to 69.9 h. The thermal stability increased five times upon immobilization.

Keywords
Candida rugosa; Mole ratio of cosurfactant/surfactant (P0); Lipase; Esterification; Microemulsion based organogel (MBG); Triton-X100
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Candida rugosa lipase immobilized in Triton-X100 microemulsion based organogels (MBGs) for ester synthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 1, January 2008, Pages 70–75
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us