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High degree polymerized chitooligosaccharides synthesis by chitosanase in the bulk aqueous system and reversed micellar microreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
35919 45114 2008 7 PDF Available
Title
High degree polymerized chitooligosaccharides synthesis by chitosanase in the bulk aqueous system and reversed micellar microreactors
Abstract

The chitosanase of Bacillus cereus NTU-FC-4 was used for the production of high degree polymerized (DP) chitooligosaccharides. In the aqueous system, the chitosanase was found to be able to catalyze a reaction to yield small amount of octamer from a mixture consisting of dimer, trimer, tetramer, pentamer, hexamer and heptamer, possibly through the transglycosylation reaction. To enhance the possible transglycosylation reaction, the process was carried out in the reversed micellar microreactors formed by AOT (sodium bis-2-(ethylhexyl) sulfosuccinate) in isooctane, and the formation of high DP chitooligosaccharides was significantly increased. It was found that the water content in the reverse micelles was an important factor affecting the enzymatic reaction. When the molar ratio of water to surfactant (W0) was 11.86, the reaction yielded the highest amount of heptamer, octamer, and nonamer. Hydrolysis of pure chitooligosaccharides by the chitosanase revealed that pentamer and hexamer might be the main gylcosyl acceptors during enzymatic reaction.

Keywords
Bacillus cereus; Chitosanase; Chitooligosaccharides; Enzymatic reaction; Reverse micelles; Microreactor
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High degree polymerized chitooligosaccharides synthesis by chitosanase in the bulk aqueous system and reversed micellar microreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 43, Issue 1, January 2008, Pages 76–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us