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Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column

Paper ID Volume ID Publish Year Pages File Format Full-Text
35939 45115 2006 5 PDF Available
Title
Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column
Abstract

The objective of this work was to apply low cost materials, agricultural residues, to the purification of xylanase. The results showed that crude extracellular, cellulase-free xylanase of an alkaliphilic Bacillus sp. strain K-8 could be purified in a single step by affinity adsorption–desorption on a corn husk column using a high flow rate, under the conditions 25 mM acetate buffer, pH 4.0, 4 °C, which prevented the hydrolysis of xylan by xylanase. After adsorption, the xylanase was eluted from the enzyme–corn husk complex with 500 mM Urea. The enzyme was purified 5.3-fold to homogeneity from culture supernatant. The molecular weight of the purified enzyme was 24 kDa as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity and recovery yield after purification were 25.4 U/mg protein and 42.3%, respectively.

Keywords
Affinity adsorption–desorption; Alkaliphilic Bacillus sp.; Corn husk column; Enzyme–insoluble substrate complex; Enzyme purification; Xylanase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 12, December 2006, Pages 2441–2445
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us