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Paclitaxel uptake and transport in Taxus cell suspension cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
3594 177 2012 7 PDF Available
Title
Paclitaxel uptake and transport in Taxus cell suspension cultures
Abstract

The transport of paclitaxel in Taxus suspension cultures was studied with a fluorescence analog of paclitaxel (Flutax-2®) in combination with flow cytometry detection. Experiments were carried out using both isolated protoplasts and aggregated suspension cell cultures. Flutax-2® was shown to be greater than 90% stable in Taxus suspension cultures over the required incubation time (24 h). Unlabeled paclitaxel was shown to inhibit the cellular uptake of Flutax-2®, although structurally similar taxanes such as cephalomannine, baccatin III, and 10-deacetylbaccatin III did not inhibit Flutax-2® uptake. Saturation kinetics of Flutax-2® uptake was demonstrated. These results indicate the presence of a specific transport system for paclitaxel. Suspension cells elicited with methyl jasmonate accumulated 60% more Flutax-2® than unelicited cells, possibly due to an increased cellular storage capacity following methyl jasmonate elicitation. The presence of a specific mechanism for paclitaxel transport is an important first result that will provide the basis of more detailed studies as well as the development of targeted strategies for increased paclitaxel secretion to the extracellular medium.

► Paclitaxel transport was studied via a fluorescent analog Flutax-2®. ► Flutax-2® is stable in suspension culture and cell-free media. ► Flutax-2® uptake was inhibited by paclitaxel but not similar taxanes. ► Saturation kinetics of Flutax-2® uptake was demonstrated. ► Methyl jasmonate increases uptake by 60%.

Keywords
Paclitaxel; Plant cell culture; Methyl jasmonate; Metabolite transport; Diffusion; Product inhibition
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 63, 15 April 2012, Pages 50–56
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us