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Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent

Paper ID Volume ID Publish Year Pages File Format Full-Text
35950 45116 2007 7 PDF Available
Title
Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent
Abstract

Haploid Saccharomyces cerevisiae W303-1A cells grown on different carbon sources were employed as the biocatalyst for ethyl acetoacetate reduction in n-hexane. The effects of cell immobilization on montmorillonite, as well as the addition of trehalose or sucrose solutions, were also tested. Best conversions (∼50%) to the chiral alcohol ethyl (S)-(+)-3-hydroxybutanoate (ee > 99%) were obtained with cells grown under respiratory metabolism with glycerol–ethanol, and higher yields were observed when trehalose was added to the reaction media. Although cells with fermentative metabolism grown on glucose were able to reduce the substrate when sucrose was added, the disaccharide was consumed by the cells during the course of the reaction, and no enantioselective product was obtained. Immobilized cells also required the addition of trehalose in order to reduce the substrate with high yield. Thus, our results indicate that trehalose may be an efficient protector of immobilized or free yeast cells during enantioselective reductions in organic solvent.

Keywords
Enantioselective reduction; Wild type Saccharomyces cerevisiae; Ethyl acetoacetate; Cell protection; Organic media; Trehalose
First Page Preview
Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 2, February 2007, Pages 141–147
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering