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Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-l-lysine microcapsule

Paper ID Volume ID Publish Year Pages File Format Full-Text
35953 45116 2007 8 PDF Available
Title
Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-l-lysine microcapsule
Abstract

A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-l-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.

Keywords
Hybridoma cell growth; Microcapsule; Modeling; Microenvironmental restrictions; Microcapsule membrane effects; Osmotic pressure
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Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-l-lysine microcapsule
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 2, February 2007, Pages 167–174
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us