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Molecular diversity and anammox activity of novel planctomycete-like bacteria in the wastewater treatment system of a full-scale alcohol manufacturing plant

Paper ID Volume ID Publish Year Pages File Format Full-Text
35955 45116 2007 8 PDF Available
Title
Molecular diversity and anammox activity of novel planctomycete-like bacteria in the wastewater treatment system of a full-scale alcohol manufacturing plant
Abstract

In this study, the diversity of planctomycete-like bacteria and the presence of anaerobic ammonia-oxidizing bacteria in the aerobic and anaerobic basins of a full-scale alcohol manufacturing wastewater treatment plant were examined by cloning and sequencing of PCR-amplified partial 16S rRNA genes. Two clone libraries were constructed by using a 16S rRNA-targeted universal reverse primer and a forward PCR primer specific for planctomycetes. Phylogenetic analysis of 63 16S rRNA gene sequences defined 52 operational taxonomic units (OTUs). The majority of these sequences (51.6%) clustered in two separated monophyletic groups distantly within the planctomycete tree. 15.9% sequences were related to some uncultured planctomycetes and branched as a monophyletic cluster from the described four genera (planctomyces, Pirellula, Isosphaera and Gemmata) of Planctomycetales with similarity of 81–89% to the cultured Planctomyces. More interestingly, 17.5% sequences (most retrieved from the anaerobic basin) formed a separated group close to the anammox cluster with similarity of 82–90% to the described anaerobic ammonia-oxidizing species despite of the unsuitable environments for these bacteria in the wastewater treatment plant. However, the following batch experiment results indicated low level of anammox activity (anaerobic ammonia-oxidizing rate of 0.8 mg NH4+-N g−1 dry biomass day−1) existing in the anaerobic biomass of alcohol manufacturing wastewater treatment plant.

Keywords
Planctomycete; Anammox activity; Anaerobic ammonia-oxidizing; Wastewater treatment; Alcohol manufacturing plant
First Page Preview
Molecular diversity and anammox activity of novel planctomycete-like bacteria in the wastewater treatment system of a full-scale alcohol manufacturing plant
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 2, February 2007, Pages 180–187
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering