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Spore production in solid-state fermentation of rice by Clonostachys rosea, a biopesticide for gray mold of strawberries

Paper ID Volume ID Publish Year Pages File Format Full-Text
35969 45116 2007 4 PDF Available
Title
Spore production in solid-state fermentation of rice by Clonostachys rosea, a biopesticide for gray mold of strawberries
Abstract

Gray mold caused by Botrytis cinerea is an important disease of strawberry. Clonostachys rosea is a mycoparasite of B. cinerea that reduces fruit losses when used as a biocontrol agent. Since spore production by C. rosea has not been optimized, we investigated factors affecting sporulation under aseptic conditions on white rice grains. The greatest spore production in glass flasks, 3.4 × 109 spores/g-dry-matter (gDM), occurred with an initial moisture content of 46% (w/w wet basis), inoculated with 1 × 106 spores/gDM and hand shaken every 15 days. However, a lower inoculum density (9 × 103 spores/gDM) and no shaking also gave acceptable sporulation. In plastic bags 1.1 × 108 spores/gDM were produced in 15 days, suggesting that larger scale production may be feasible: with this spore content, 24 m2 of incubator space would produce sufficient spores for the continued treatment of 1 ha of strawberry plants.

Keywords
Clonostachys rosea; Botrytis cinerea; Biological control; Grey mould; Strawberry
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Spore production in solid-state fermentation of rice by Clonostachys rosea, a biopesticide for gray mold of strawberries
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 2, February 2007, Pages 275–278
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us