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Improved performance of a packed-bed reactor for biodiesel production through whole-cell biocatalysis employing a high-lipase-expression system

Paper ID Volume ID Publish Year Pages File Format Full-Text
3597 177 2012 5 PDF Available
Title
Improved performance of a packed-bed reactor for biodiesel production through whole-cell biocatalysis employing a high-lipase-expression system
Abstract

To improve enzymatic biodiesel production, we developed a packed-bed reactor (PBR) system using recombinant cells, into which a strong enolase promoter and 5′ untranslated region were introduced. Aspergillus oryzae expressing Fusarium heterosporum lipase was immobilized within biomass support particles (BSPs) during cultivation and used directly as a whole-cell biocatalyst. BSP-immobilized A. oryzae carrying three copies of the expression cassette showed a higher performance than previously developed cell systems, resulting in 87.5% conversion after 10 passes in PBR. The performance was also affected by operational factors including residence time and methanol feeding. After optimization, the PBR system attained a final methyl ester content of 96.1% with a residence time of 140 min per pass and stepwise addition of 4.25 molar equivalents of methanol to oil for 6 passes. Moreover, lipase activity was maintained for 5 batch cycles. Therefore, the developed PBR employing a high-lipase-expression system is considered useful for improving enzymatic biodiesel production.

► A packed-bed reactor system was developed using recombinant fungal whole-cells. ► We used immobilized Aspergillus oryzae employing a high-lipase-expression system. ► Optimization enabled the achievement of a methyl ester content above 96%. ► The batch methanolysis could be repeated for 5 cycles.

Keywords
Biodiesel fuel; Methanolysis; Filamentous fungi; Immobilized cells; Lipase; Packed-bed reactor
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Improved performance of a packed-bed reactor for biodiesel production through whole-cell biocatalysis employing a high-lipase-expression system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 63, 15 April 2012, Pages 76–80
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us