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Studies on adsorption isotherms of endotoxin and BSA using an affinity column

Paper ID Volume ID Publish Year Pages File Format Full-Text
35971 45116 2007 4 PDF Available
Title
Studies on adsorption isotherms of endotoxin and BSA using an affinity column
Abstract

In this paper, an adsorbent with dimethylamine ligand for endotoxin removal was prepared and used to study the adsorption isotherms of endotoxin and BSA. The results showed that as the introducing of endotoxin, the maximum adsorption capacity qm of BSA increased from 7.24 to 7.74 mg/mL and the apparent association constant KA of BSA decreased from 14.06 to 11.48 mL/mg. The adsorption isotherms of BSA changed from Langmuir model to Multilayer model. All these gave the direct evidences to construct the adsorption process. By comparing adsorption isotherms of endotoxin with BSA, it was found that the apparent association constant KA of endotoxin was much higher than that of BSA.

Keywords
Endotoxin; BSA; Adsorption isotherm; Agarose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 2, February 2007, Pages 285–288
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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