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Aureobasidium pullulans batch cultivations based on a factorial design for improving the production and molecular weight of exopolysaccharides

Paper ID Volume ID Publish Year Pages File Format Full-Text
35984 45117 2007 8 PDF Available
Title
Aureobasidium pullulans batch cultivations based on a factorial design for improving the production and molecular weight of exopolysaccharides
Abstract

Six factors: strain, carbon source, nitrogen source, nitrogen concentration, aeration, and initial pH, were investigated for their effects on exopolysaccharides (EPS) production in Erlenmeyer flasks by Aureobasidium pullulans using 2-level fractional factorial design. The concentration and molecular weight (MW) of EPS were optimized simultaneously. The effects of main factors together with possible two-factor interactions were detected, and the levels of the six factors were optimized using empirical models. Analysis of factor effects revealed that strain had the strongest influence on EPS concentration, and nitrogen source on MW of EPS. However, nitrogen concentration did not show significant influence on both parameters. The influences of factors on the production variability were also monitored for quality control purposes. At the optimum levels of control factors investigated, as high as 22.6 g/L EPS with a weight average MW of higher than 2 × 106 was produced. The results of this work indicate that the production kinetics varies for different microorganism–medium–environment systems.

Keywords
Aureobasidium pullulans; Pullulan fermentation; Molecular weight; Experimental design; Optimization; Shake flask culture
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Aureobasidium pullulans batch cultivations based on a factorial design for improving the production and molecular weight of exopolysaccharides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 820–827
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us