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Cell cycle model for recombinant Pichia pastoris during glycerol fed-batch cultivation

Paper ID Volume ID Publish Year Pages File Format Full-Text
35985 45117 2007 6 PDF Available
Title
Cell cycle model for recombinant Pichia pastoris during glycerol fed-batch cultivation
Abstract

A cell cycle model is proposed for methylotrophic yeast Pichia pastoris grown on glycerol during fed-batch cultivation. Morphological differentiation of cells, such as unbudded daughter cell, unbudded parent cell and budding cell, is depicted by the model. During the cyclic growth, cells in different cycling period are assumed to undergo sequential shifting dominantly. The input of the cell cycle model is the specific growth rate, which is calculated from the macrokinetic model proposed previously. The cell cycle related variables, such as the fraction of budding cells and the cell density are then simulated. Model validation is carried out with the experimental data of off-line assays.

Keywords
Pichia pastoris; Cell cycle model; Morphology; Specific growth rate; Cell density; Fraction of budding cells
First Page Preview
Cell cycle model for recombinant Pichia pastoris during glycerol fed-batch cultivation
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 828–833
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering