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Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
35992 45117 2007 6 PDF Available
Title
Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods
Abstract

Medium compositions for the production of the novel derivatives of FR-008/Candicidin which was produced by a mutant of Streptomyces sp. FR-008 were optimized using two statistical methods including Plackett–Burman design (P–B), which was applied to find the key ingredients for the best medium composition, and response surface methodology (RSM), which was used to determine the optimal concentrations of these components. Results indicated that peptone, copper sulfate and glycerol had significant effects on the production. Under the proposed optimized conditions, the CS101 experimental yield (191.259 mg/L) closely matched the yield (203.536 mg/L) predicted by the statistical model. The optimization of the medium contributed to 10-fold higher antibiotic production than that of the control. It was first revealed that copper could stimulate FR-008/Candicidin and their derivatives synthesis at an optimal concentration in this paper, moreover, the basis of this phenomenon was also explained by investigating the enhancement of the enzymatic pathways.

Keywords
Novel antibiotic; FR-008/Candicidin derivatives; Optimization; Plackett–Burman design; Response surface methodology; Enzymatic pathways
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Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 878–883
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us