fulltext.study @t Gmail

Precursor-feeding strategy for the production of solanine, solanidine and solasodine by a cell culture of Solanum lyratum

Paper ID Volume ID Publish Year Pages File Format Full-Text
35996 45117 2007 5 PDF Available
Title
Precursor-feeding strategy for the production of solanine, solanidine and solasodine by a cell culture of Solanum lyratum
Abstract

Solanum lyratum, a medicinal plant, has been used to treat cancers, tumors, and warts for many years. Undifferentiated cell cultures were mainly used to study the precursor-feeding strategy for the production of secondary metabolites of α-solanine, solanidine, and solasodine for pharmaceutical usage. In this study, S. lyratum cells were fed with exogenous plant sterols including cholesterol, stigmasterol, and mixed sterols (β-sitosterol, campesterol, and dihydrobrassicasterol). The results showed that none of the plant sterols exhibited an effect on cell growth as compared to that of the control. Cellular concentrations of solanidine and solasodine were relatively higher than α-solanine levels in all the treatments. The maximal solasodine level in cells was 11.19 mg/g dry weight (DW) after 0.05–1 mg/l stigmasterol feeding, which was about 10-fold higher than the control. With regard to solanidine levels, the maximal level in cells was 5.84 mg/g DW after feeding with 20 mg/l cholesterol. This is the first report on the in vitro enhancement of solanidine and solasodine, steroidal alkaloids with medicinal value, from S. lyratum.

Keywords
2,4-D, 2,4-dichlorophenoxyacetic acidSolanum lyratum; Precursor feeding; Steroidal alkaloid; Solanine; Solanidine; Solasodine
First Page Preview
Precursor-feeding strategy for the production of solanine, solanidine and solasodine by a cell culture of Solanum lyratum
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 899–903
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us