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The effects of organic solvent/water mixtures on the structure and catalytic activity of porcine pepsin

Paper ID Volume ID Publish Year Pages File Format Full-Text
35998 45117 2007 4 PDF Available
Title
The effects of organic solvent/water mixtures on the structure and catalytic activity of porcine pepsin
Abstract

The effects of aqueous solutions of ethanol, acetonitrile and 1,4-dioxane in the concentration range 10–90% (v/v) on the activity of porcine pepsin were studied. The enzyme retained its activity in aqueous ethanol and aqueous acetonitrile with increasing organic solvent concentration up to 60%, and in aqueous 1,4-dioxane up to 30%, but thereafter a considerable decrease in activity was observed. The changes caused in the catalytic activity by the water-miscible organic solvents may be related to structural changes, which were followed by means of intrinsic fluorescence and circular dichroism spectroscopy measurements.

Keywords
Porcine pepsin; Enzyme activity; Aqueous organic solvents; Intrinsic fluorescence; CD spectroscopy
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The effects of organic solvent/water mixtures on the structure and catalytic activity of porcine pepsin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 909–912
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us