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Fractionation and characterization of chlorophyll and lignin from de-juiced Italian ryegrass (Lolium multifolrum) and timothy grass (Phleum pratense)

Paper ID Volume ID Publish Year Pages File Format Full-Text
35999 45117 2007 6 PDF Available
Title
Fractionation and characterization of chlorophyll and lignin from de-juiced Italian ryegrass (Lolium multifolrum) and timothy grass (Phleum pratense)
Abstract

In this study, de-juiced Italian ryegrass and timothy grass were successfully fractionated into one chlorophyll rich fraction, two lignin fractions, two hemicellulosic preparations, and one cellulosic fraction by sequential processes with 80% ethanol containing 0.2% NaOH, 2.5% H2O2–0.2% EDTA containing 1.5% NaOH, and 2.5% H2O2–0.2% TAED containing 1.0% NaOH at 75 °C for 3 h, respectively. The yields of chlorophyll rich fraction and total lignin fraction were found to be 6.1 and 11.0% from de-juiced Italian ryegrass, and 12.7 and 13.2% from de-juiced timothy grass. It should be noted that the two chlorophyll rich fractions contained noticeable amounts of contaminated hemicelluloses (17.1–18.8%) and lignin, whereas the four lignin fractions contained only small amounts of bound hemicelluloses (5.6–8.6%). Nitrobenzene oxidation and NMR spectra of the lignin fraction revealed that the presence of both guaiacyl and syringyl units, as well as p-hydroxyphenyl structures. Small amounts of esterified p-coumaric acids and mainly etherified ferulic acids were also identified in the lignin fractions. It was found that the lignin fractions obtained from the two different grasses had very similar structural composition. They are distinguished by rather low amounts of β-O-4 structures, resinol units (β-β), and of condensed units (β-5 and 5-5). This trait is even more pronounced in the production of phenolic compounds for chemical industry from grass.

Keywords
Italian ryegrass; Timothy grass; Fractional process; Lignin; Chlorophyll
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Fractionation and characterization of chlorophyll and lignin from de-juiced Italian ryegrass (Lolium multifolrum) and timothy grass (Phleum pratense)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 913–918
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us