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Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
36004 45118 2007 9 PDF Available
Title
Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor
Abstract

An alkaline lipase from Burkholderia multivorans was produced within 15 h of growth in a 14 L bioreactor. An overall 12-fold enhanced production (58 U mL−1 and 36 U mg−1 protein) was achieved after medium optimization following the “one-variable-at-a-time” and the statistical approaches. The optimal composition of the lipase production medium was determined to be (% w/v or v/v): KH2PO4 0.1; K2HPO4 0.3; NH4Cl 0.5; MgSO4·7H2O 0.01; yeast extract 0.36; glucose 0.1; olive oil 3.0; CaCl2 0.4 mM; pH 7.0; inoculum density 3% (v/v) and incubation time 36 h in shake flasks. Lipase production was maximally influenced by olive oil/oleic acid as the inducer and yeast extract as the additive nitrogen. Plackett–Burman screening suggested catabolite repression by glucose. Amongst the divalent cations, Ca2+ was a positive signal while Mg2+ was a negative signal for lipase production. RSM predicted that incubation time, inoculum density and oil were required at their higher levels (36 h, 3% (v/v) and 3% (v/v), respectively) while glucose and yeast extract were required at their minimal levels for maximum lipase production in shake flasks. The production conditions were validated in a 14 L bioreactor where the incubation time was reduced to 15 h.

Keywords
Burkholderia multivorans; Lipase; Statistical designing; Medium optimization; Plackett–Burman Design; Response Surface Methodology
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Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 4, April 2007, Pages 518–526
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us