fulltext.study @t Gmail

Separation of β22 dimer from bovine bone collagen

Paper ID Volume ID Publish Year Pages File Format Full-Text
36007 45118 2007 5 PDF Available
Title
Separation of β22 dimer from bovine bone collagen
Abstract

The precise role of the α2-chain in collagen type I is of considerable scientific interest. Our recent studies demonstrated that the most noticeable difference between type I collagens, which were obtained from bovine hard tissues (bone, dentine) and soft tissues (tendon, skin), was presented in the position of β chain dimers using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The additional band observed both in the bone and dentine collagen was putatively identified as β22 dimer (made of by an intermolecular cross-linking between two α2-chains). Further investigations carried out on bovine bone and skin collagen, corresponding to hard tissue and soft tissue collagen respectively, confirmed this hypothesis. Successful separation of individual β22 dimer from bone collagen was achieved. The procedure involves molecular-sieve chromatography on a Sephacryl S-400 column followed by differential acetone precipitation. Identification was done by the widely used methods, such as SDS-PAGE and cyanogen bromide (CNBr)-cleaved peptide analysis. It was proposed that the dimer and consequently α2-chains may play important roles in the morphological and biological differences between hard and soft tissues.

Keywords
SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; CNBr, cyanogen bromide; CHCl3, chloroform; CH3OH, methanol; GuHCl, guanidine hydrochlorideBone collagen; Intermolecular cross-link; β22 dimer; α2-Chain
First Page Preview
Separation of β22 dimer from bovine bone collagen
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 4, April 2007, Pages 542–546
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us