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A comparison of disruption procedures for the analysis of phospholipids from Streptomyces pristinaespiralis

Paper ID Volume ID Publish Year Pages File Format Full-Text
36029 45118 2007 4 PDF Available
Title
A comparison of disruption procedures for the analysis of phospholipids from Streptomyces pristinaespiralis
Abstract

Combinations of three cell disrupting methods were used for lipid extraction from the Gram-positive bacterium, Streptomyces pristinaespiralis: lysozyme treatment, freeze-drying and bead mill. The determination of extracted phospholipids (PL) was carried out using thin layer chromatography coupled with a flame ionisation detector. It appeared that freeze-drying induced the highest PL extraction and that only lysozyme addition permitted to extract phosphatidylserine. The optimal cellular dry weight for the extraction and the subsequent separation and analysis of the PL was 50 mg.

Keywords
DPG, diphosphatidylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserineLipid extraction; Membrane phospholipids; TLC-FID; Streptomyces
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A comparison of disruption procedures for the analysis of phospholipids from Streptomyces pristinaespiralis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 4, April 2007, Pages 700–703
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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