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Galanthamine production by Leucojum aestivum in vitro systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
36035 45118 2007 6 PDF Available
Title
Galanthamine production by Leucojum aestivum in vitro systems
Abstract

The callus induction from young fruits of Leucojum aestivum was performed on Murashige–Skoog nutrient medium supplemented with 4 mg/L 2,4 dichlorphenoxyacetic and 2 mg/L 6-benzylamynopurine. Further, by planting the obtained calluses on the same nutrient medium supplemented with 1.15 mg/L α-naphtylacetic acid and 2.0 mg/L 6-benzylamynopurine shoot cultures were established. The growth and galanthamine accumulation of obtained L. aestivum in vitro systems were studied. It was established that the amount of accumulated galanthamine strongly depended on the level of the differentiation. The maximum yield of biomass (17.8 g/L) and the maximum amount of accumulated galanthamine (2.5 mg/L) were achieved after day 35 of submerged cultivation of L. aestivum 80 shoot culture, performed under illumination. Data concerning the time courses of the utilization of the main nutrient components of the medium during cultivation of L. aestivum shoot culture are presented as well.

Keywords
Alkaloids; Amaryllidacea; Callus culture; Galanthamine; Leucojum aestivum; Shoot culture
First Page Preview
Galanthamine production by Leucojum aestivum in vitro systems
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 4, April 2007, Pages 734–739
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering