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Optimizing lipase production of Burkholderia sp. by response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
36042 45119 2006 5 PDF Available
Title
Optimizing lipase production of Burkholderia sp. by response surface methodology
Abstract

This work utilized experimental design methodology to improve lipase production from an indigenous bacterial isolate identified as Burkholderia sp. C20. The factors affecting the lipase production of Burkholderia sp. C20 were screened by two-level factorial design and the optimal conditions for lipase production were determined by response surface methodology (RSM). Preliminary batch tests were employed to obtain the favorable conditions for lipase activity analysis and found that the optimal temperature and pH for lipase activity assay was 55 °C and 9.0, respectively. Comparison of cell growth and lipase activity profiles shows that the fermentation time of 67 h was suitable for harvesting lipase products in the batch culture of Burkholderia sp. C20. A two-level design of 25−1 experiments was applied to identify the most significant influential factors out of five factors; namely, temperature, pH, and concentrations of olive oil, CASO and NaCl. After analysis with two-level design, concentrations of CASO and NaCl were selected for RSM analysis, predicting an optimal composition of 0.12% and 0.16% for CASO and NaCl, respectively. Using the optimal conditions, lipase production by Burkholderia sp. C20 was enhanced nearly 5 fold (from 0.8 to 3.9 U/ml).

Keywords
Lipase; Burkholderia sp. C20; Response surface methodology (RSM); Two-level factorial design
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 9, September 2006, Pages 1940–1944
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us