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Purification and characterization of a novel stable ginsenoside Rb1-hydrolyzing β-d-glucosidase from China white jade snail

Paper ID Volume ID Publish Year Pages File Format Full-Text
36047 45119 1980 7 PDF Available
Title
Purification and characterization of a novel stable ginsenoside Rb1-hydrolyzing β-d-glucosidase from China white jade snail
Abstract

A highly stable β-glucosidase from the viscera of China white jade snail was purified to apparent homogeneity. This purified glucosidase consisted of two identical subunits with a native molecular mass of approximately 230 kDa. The maximal activity to p-nitrophenyl-β-d-glucopyranoside (pNPG) occurred at 70 °C and pH 5.6. Its most notable characteristic was stable over a wide pH range (3–11 at 30 °C for 24 h) and a relatively high temperature (60 °C for 24 h). The Km for pNPG was 0.338 mM and the Vmax was 0.25 mmol nitrophenol/min/mg glucosidase at pH 5.6 and 50 °C. Compared to its activity against pNPG (100%), the β-glucosidase exhibited low levels of activity against other aryl-glycosides (less than 1%). Additionally, the enzyme hydrolyzed the 20-C, β-(1 → 6)-glucoside of ginsenoside Rb1 to produce ginsenoside Rd, but did not hydrolyze the other β-d-glucosidic bonds of Rb1. The properties of the enzyme could make it become a useful tool in biotransformation of glucosides.

Keywords
β-d-Glucosidase; Thermo- and pH-stability; Ginsenoside Rb1; Ginsenoside Rd; Purification; China white jade snail
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Purification and characterization of a novel stable ginsenoside Rb1-hydrolyzing β-d-glucosidase from China white jade snail
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 9, September 2006, Pages 1974–1980
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us