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Nocardia globerula NHB-2: Bench scale production of nicotinic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
36062 45119 2006 4 PDF Available
Title
Nocardia globerula NHB-2: Bench scale production of nicotinic acid
Abstract

The intracellular nitrilase of Nocardia globerula NHB-2 induced by propionitrile catalyses the hydrolysis of 3-cyanopyridine to nicotinic acid without detectable formation of nicotinamide. N. globerula NHB-2 showed highest conversion rate at 40 mM 3-cyanopyridine, in 0.1 M NaH2PO4/Na2HPO4 buffer (pH 7.5) containing 5 mM 2-mercaptoethanol. In 1 l fed-batch reaction, containing 0.1 M NaH2PO4/Na2HPO4 buffer (pH 7.5), 4.2 g resting cells (dry weight), at 35 °C, 400 rpm, a total of 1 mol of 3-cyanopyridine added at feed rate of 0.12 mol h−1 was converted to nicotinic acid (98.6%) in 9 h. The rate of nicotinic acid production was 26 mmol h−1 g−1 DW.

Keywords
3-Cyanopyridine; Nicotinic acid; Nitrilase; Nocardia globerula NHB-2; Propionitrile
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 9, September 2006, Pages 2078–2081
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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