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Novel application of simulated moving bed chromatography to protein refolding

Paper ID Volume ID Publish Year Pages File Format Full-Text
36077 45120 2006 11 PDF Available
Title
Novel application of simulated moving bed chromatography to protein refolding
Abstract

A new continuous refolding method using a four-zone simulated moving bed process based on the size exclusion mechanism was developed in order to overcome the disadvantages associated with inclusion body refolding in a batch dilution and chromatography. Protein refolding using size exclusion SMB enables refolded protein to be obtained continuously with a high productivity, low consumption of the refolding buffer, and a high efficiency of the size exclusion medium. The size exclusion factors and kinetic parameters for the SMB operation were estimated from the best-fit values by comparing the simulation and experimental chromatography results. The SMB operating conditions were obtained from the standing wave design. Computer simulations and several SMB experiments showed that the standing wave design and the proposed process could achieve a high level of protein recovery (96%), a high specific protein refolding yield (96%), and low degree of protein aggregation.

Keywords
Lysozyme; Continuous protein refolding; Size-exclusion chromatography; Simulated moving bed chromatography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 5, May 2006, Pages 1072–1082
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us