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Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel as inducer

Paper ID Volume ID Publish Year Pages File Format Full-Text
36086 45120 2006 5 PDF Available
Title
Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel as inducer
Abstract

Different alginate systems, viz., sodium alginate, glutaraldehyde-treated alginate and PVA-alginate gel were examined for the immobilization of whole cells of Aspergillus niger. The enzyme activity of sodium alginate immobilized cells was greater as compared to the other two systems. In the process, dried orange peel powder served as a very good inducer of PGase. Maximum enzyme production was observed with 3% alginate and 1.5% orange peel inducer in 72 h; the ratio of gel beads/medium (v/v) being 1:4. The immobilized beads could be effectively reused three times with a loss of 5% activity. After the third cycle, there was a loss of 3% with each consecutive use till the sixth cycle. The present immobilization system indicates the possibility of its application in a semi-continuous process for the production of polygalacturonase.

Keywords
Polygalacturonase; Production; Aspergillus niger; Orange peel; Immobilization; Alginate; Reusability
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Production of polygalacturonase by immobilized cells of Aspergillus niger using orange peel as inducer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 5, May 2006, Pages 1136–1140
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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