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Optimization of medium composition for the production of transglutaminase by Bacillus circulans BL32 using statistical experimental methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
36094 45120 2006 7 PDF Available
Title
Optimization of medium composition for the production of transglutaminase by Bacillus circulans BL32 using statistical experimental methods
Abstract

Medium composition for transglutaminase (MTGase) production by Bacillus circulans BL32, a recently isolated strain from the Amazon basin, was optimized using a stepwise strategy. Effects of carbon, nitrogen and mineral sources were investigated by screening experiments. Plackett–Burman (P–B) statistical design was applied to find the key ingredients for the best medium composition among glycerol, sucrose, peptone, tryptone, Na2HPO4, MgSO4·7H2O and FeSO4·7H2O. Results indicated that sucrose, negatively, and peptone, positively, have significant effects on MTGase production, with confidence levels above 90%. Central composite design (CCD) was used to determine the optimal concentrations of these two components and the experimental results were fitted with a second-order polynomial model at 90% level (P < 0.10). Under the proposed optimized conditions, the model predicted the MTGase activity of 0.280 U/mL, very closely matching experimental activities of 0.306 U/mL.

Keywords
Bacillus circulans BL32; Medium optimization; Microbial transglutaminase; Plackett–Burman design; Response surface methodology
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Optimization of medium composition for the production of transglutaminase by Bacillus circulans BL32 using statistical experimental methods
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 5, May 2006, Pages 1186–1192
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us