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Characterizations of immobilized neutral proteinase on chitosan nano-particles

Paper ID Volume ID Publish Year Pages File Format Full-Text
36095 45120 2006 5 PDF Available
Title
Characterizations of immobilized neutral proteinase on chitosan nano-particles
Abstract

In order to investigate the characterizations of chitosan nano-particles prepared by ionization gelation method, neutral proteinase was selected as a model enzyme to assess its potential applicability for the enzyme immobilization. The characterizations of immobilized enzyme were studied. The highest enzyme activity of immobilized enzyme was allowed at pH 7.5, identical to that of free enzyme, but the range of the optimum pH was broadened. The optimal temperature of immobilized enzyme was 55 °C, and the range of the optimum temperature was also broadened. The thermal, operational and storage stabilities of immobilized enzyme were improved after they were immobilized on chitosan nano-particles. Chitosan nano-particles were applied to protect the activity of neutral proteinase. They could improve 13.17% of neutral proteinase activity than that of free neutral proteinase at the same conditions. The Michaelis constant Km for immobilized enzyme was disclosed to 0.6629 mg/ml by Line weaver-Burk plot at 40 °C.

Keywords
Chitosan; Nano-particles; Characterizations; Enzyme; Immobilization
First Page Preview
Characterizations of immobilized neutral proteinase on chitosan nano-particles
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 5, May 2006, Pages 1193–1197
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering