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Production, purification and partial characterization of a novel endo-β-1,3-glucanase from Agaricus brasiliensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
36102 45120 2006 5 PDF Available
Title
Production, purification and partial characterization of a novel endo-β-1,3-glucanase from Agaricus brasiliensis
Abstract

An endo-β-1,3-glucanase from Agaricus brasiliensis ATCC 76739 was first produced and purified in the submerged culture. Purified endo-β-1,3-glucanase with a 13.1-fold purification and 6.7% yield was prepared by two steps: precipitation with 70% saturation ammonium sulfate and chromatography on hydrophobic interaction chromatography. The molecular mass of the enzyme was estimated to be 33 kDa by SDS-PAGE. The presence of endo-β-1,3-glucanase might explain the fall of the yield of bioactive exopolysaccharides in the late stage of the submerged cultures. The pH optimum for the enzyme was 4.5, and the temperature optimum was 45 °C. The enzyme showed high pH stability within the range of pH 3.5–6.0 and thermostability up to 50 °C, and exhibited a half-life of 30 min at 55 °C, which was better than that of a thermophilic fungus, Scytalidium thermophilum. The enzyme activity was strongly inhibited by HgCl2.

Keywords
Agaricus brasiliensis; Endo-β-1,3-glucanase; Purification; Exopolysaccharides; Thermostable; Characterization
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Production, purification and partial characterization of a novel endo-β-1,3-glucanase from Agaricus brasiliensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 5, May 2006, Pages 1229–1233
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us