fulltext.study @t Gmail

Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
36107 45121 2006 6 PDF Available
Title
Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation
Abstract

Rhizopus homothallicus (IRD13a) was cultivated for lipase production in solid state fermentation (SSF) using sugarcane bagasse as a support and impregnated with a liquid medium. The production of an extracellular lipase from Rh. homothallicus was improved by modification of media nutrients. The nutrients that mainly influenced growth and lipase production were urea, olive oil and oligo-elements. Kinetics of growth and lipase production were carried out using the improved medium. A correlation was found between the lipase production profile and the profiles of CO2 production, O2 consumption and pH changes. A maximum of 826 U/g DM was reached in only 12 h of incubation. This production represented a 60-fold increase of lipolytic activity compared to that obtained using a well known medium for lipase production. These results are promising because this strain produces high lipase concentrations in an inexpensive and simple medium, which facilitates its recovery and purification. Lipase extraction from the fermented solid was also studied. Efficient recovery of the enzyme was achieved using Triton X-100 at 0.5% (w/v).

Keywords
Rhizopus homothallicus; Solid state fermentation; Lipase; Enzyme extraction
First Page Preview
Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 11, November 2006, Pages 2264–2269
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us