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The inhibitory effects of freshwater clam (Corbicula fluminea, Muller) muscle protein hydrolysates on angiotensin I converting enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
36109 45121 2006 6 PDF Available
Title
The inhibitory effects of freshwater clam (Corbicula fluminea, Muller) muscle protein hydrolysates on angiotensin I converting enzyme
Abstract

The muscle of golden freshwater clam was extracted using hot water. The residual meat was freeze-dried then hydrolyzed at 50 °C first by Protamex (PX) as primary hydrolysis followed by a secondary hydrolysis (Flavourzyme, F). The inhibitory effects of hot water extracts and hydrolysates against angiotensin I converting enzyme (ACE) were investigated. The soluble protein content and peptide content of the hot water extracts was 85 and 84 mg/g, respectively. The IC50 on ACE was 1.95 mg/ml. The highest values of soluble protein (571 mg/g) and peptide content (272 mg/g) of hydrolysate prepared by PX hydrolysis for 5 h (PX5) or PX hydrolysis for 5 h followed by F hydrolysis for 0.5 h (PX5F0.5). PX5 showed the lowest IC50 on ACE (0.043 mg/ml), and a mixed-type inhibition kinetics while Captopril showed competitive inhibition. Their Ki values were 0.032 mg/ml and 0.0067 μg/ml, respectively. The PX5 hydrolysate was fractionated by size exclusion chromatography on a Sephadex G-25. A fraction of the PX5 had molecular weight ranged 420–380 Da showed the highest inhibitory efficiency ratio (IER) being 1314.7%/mg/ml. The amino acid sequences of the two peaks of this fraction PX-D1 and PX-D2 were Val-Lys-Pro and Val-Lys-Lys, of which IC50 value were 3.7 and 1045 μM, respectively. This freshwater clam hydrolysate (PX5) (peptide concentration 5 mg/ml) was used as drinks administered to spontaneously hypertensive rats (SHR) for 8 weeks. The systolic blood pressure and diastolic blood pressure of the SHR were significantly reduced by 22.0 and 13.2 mmHg, respectively, indicating a hypotensive effect by oral administration.

Keywords
Freshwater clam; Protein hydrolysate; Angiotensin I converting enzyme; Spontaneously hypertensive rat (SHR); Antihypertensive effect
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 11, November 2006, Pages 2276–2281
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us