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Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media

Paper ID Volume ID Publish Year Pages File Format Full-Text
36112 45121 2006 8 PDF Available
Title
Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media
Abstract

As serum elimination from animal cell culture media aims at avoiding the risks associated with components of bovine origin, additives such as protein hydrolysates from plant sources are proposed. In this study, we evaluate the interest of adding various peptide mixtures, obtained by membrane fractionation of an enzymatic hydrolysate of rapeseed proteins, in CHO cell cultures. While the total hydrolysate and some fractions derived from this hydrolysate do not affect CHO cell kinetics, one fraction, composed of a mixture of both large (500–5000 Da) and small peptides (<500 Da), significantly stimulates cell growth rate (maximal cell density × 1.7) when added at 4 g/l. In addition, this fraction allows the formulation of a safe, very simple protein-free medium (RPMI + rapeseed peptides), which does not compromise cell growth in stirred conditions, strongly reduces cell death rate (culture duration time × 2), increases recombinant γ-interferon production, accelerates cell adaptation to serum-free conditions and facilitates cell cryopreservation.

Keywords
Rapeseed; Peptide fractions; Protein hydrolysate; Protein-free medium; CHO cell culture
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Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 11, November 2006, Pages 2297–2304
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us