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Genome-wide expression analyses of adaptive response against medadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377

Paper ID Volume ID Publish Year Pages File Format Full-Text
36113 45121 2006 9 PDF Available
Title
Genome-wide expression analyses of adaptive response against medadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377
Abstract

Menadione (MD), a simplest synthetic quinone used with reactive oxygen species (ROSs) generating agents, causes damages to Saccharomyces cerevisiae KNU5377. To understand antioxidant defense mechanisms under adaptive stress response, we have performed biochemical analyses and the genome-wide gene expression analysis after exposure to 120 μM of MD with and without adaptation. KNU5377 could survive at higher concentration of MD, 680 μM for 1 h, comparing with S. cerevisiae W303, ATCC24858 as reference strains. Glutathione peroxidase (GPX2), thioredoxin peroxidase 2 (TSA2), and thioredoxin reductase (TRR1) were up-regulated over 10-fold after treatment of 120 μM. Superoxide dismutase (SOD1), catalase (CTA1), glucose-6-phosphate dehydrogenase (ZWF1), glutathione peroxidase (GPX2, HYR1), glutathione reductase (GLR1), thioredoxin peroxidase (AHP1), and thioredoxin 2 (TRX2) were also strongly induced after adaptation. Additionally, transcriptional analysis of those genes was well corresponded with up-regulated protein expression and enzyme activity. Antioxidant defense mechanisms containing SOD1, ZWF and TRX2 maybe orchestrate by the different levels at the same time and function to produce effective defense systems. These results suggest that specific mechanisms including the three proteins were well organized for acquiring adaptive response to MD in this strain.

Keywords
Saccharomyces cerevisiae; Microarray; Oxidative stress; Adaptive response; Antioxidant enzymes; Gene expression
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Genome-wide expression analyses of adaptive response against medadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 11, November 2006, Pages 2305–2313
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us