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Rational development of serum-free medium for Chinese hamster ovary cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
36114 45121 2006 6 PDF Available
Title
Rational development of serum-free medium for Chinese hamster ovary cells
Abstract

Recombinant Chinese hamster ovary (CHO) cells which produce macrophage colony-stimulating factor (M-CSF) were used as a model to systematically develop the serum-free medium. Pre-weaning strategy combined with experimental design was adopted to develop the serum-free medium for CHO cells in this study. First, CHO cells were adapted to grow in a commercial serum-free medium. Then, these weaned cells were adopted to develop our own serum-free medium using experimental design. Insulin and SerEx had significantly stimulatory influence on M-CSF production (P < 0.05) among the seven screened supplements by use of fractional factorial design. Response surface methodology was consequently applied to find optimal concentrations of insulin and SerEx for M-CSF production. By virtue of the pre-weaning strategy and experimental designs, the development of serum-free medium could be simplified and accelerated.

Keywords
CHO; M-CSF; Serum-free medium; Experimental design; Pre-weaning strategy
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Rational development of serum-free medium for Chinese hamster ovary cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 11, November 2006, Pages 2314–2319
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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