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Modeling bacteriophage attachment using adsorption efficiency

Paper ID Volume ID Publish Year Pages File Format Full-Text
3612 178 2012 8 PDF Available
Title
Modeling bacteriophage attachment using adsorption efficiency
Abstract

Typically, models of bacteriophage adsorption consider the process in two steps: reversible and irreversible attachment. In this study, a recently introduced one-step adsorption model, the adsorption efficiency model, is used to describe the adsorption of T-series bacteriophages to Escherichia coli. The adsorption efficiency model simplifies phage attachment to a single step: irreversible binding. The adsorption efficiency (ɛ) is used to account for unadsorbed phages. The model accurately describes T-series phage adsorption (T2, T4, T5, T6, and T7) under a variety of conditions. In addition, the model is compared to a commonly used two-step adsorption model, the sequential model. Experimental data support the assumptions of the adsorption efficiency model and suggest that the reversible first step of T-series phage adsorption is equivalent to irreversible attachment under the conditions tested. The adsorption efficiency model was not appropriate for a phage λ strain lacking side tail fibers. However, the model did agree with data previously published for a strain of phage λ possessing side tail fibers, as is the case of all T-series strains tested. This suggests that the adsorption efficiency model applies to phages containing side tail fibers

► Demonstrates robustness of adsorption efficiency model. ► Shows the applicability of adsorption efficiency model to different phages. ► Gives experimental support for assuming reversible step in adsorption is negligible. ► Demonstrates better fit of model compared to common sequential adsorption model. ► Shows limits to model, e.g. it does not apply to phages without side tail fibers adsorption.

Keywords
Bacteriophage; Adsorption; Adsorption efficiency; Modeling; Kinetic parameters; Dynamic simulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 64, 15 May 2012, Pages 22–29
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us