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Comparison of two matrices for selective recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates

Paper ID Volume ID Publish Year Pages File Format Full-Text
36129 45122 2007 9 PDF Available
Title
Comparison of two matrices for selective recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates
Abstract

A comparative study of the performance of two types of adsorbent (Streamline Quartz Base and Upfront Matrices), derivatized with the same affinity ligand (RPAP) to recover C595 diabody fragment (dbFv) from Escherichia coli lysates has been undertaken. Both streamline and Upfront Matrices are characterized by a particle size range of 100–300 μm. Streamline has a density of 1.20 g cm−3 and ligand concentration of 0.85 μmol ml−1. Upfront has a density of 1.35 g cm−3 and ligand concentration of 0.83 μmol ml−1. The release of C595 dbFv from E. coli cells was achieved by a chemical lysis method. The recovery performance of both adsorbents was evaluated in terms of operational productivity and elution yield of C595 dbFv in packed bed (clarified feedstock) and expanded bed (unclarified and clarified feedstock) chromatography systems. Streamline and Upfront adsorbents exhibited diabody operational productivities of 131 and 202 mg l−1, respectively, with an elution yield of 92 and 94%, respectively, in packed bed operation. Streamline and Upfront adsorbents exhibited diabody operational productivities of 54.5 and 123.7 mg l−1, respectively, with an elution yield of 89 and 92%, respectively, in expanded bed operation.

Keywords
C595 dbFv; Affinity ligand; Streamline Quartz Base; Upfront matrices; Elution yield
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Comparison of two matrices for selective recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 335–343
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us