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Use of sea water as salts source in starch- and soya bean-based media, for the production of Bacillus thuringiensis bioinsecticides

Paper ID Volume ID Publish Year Pages File Format Full-Text
36133 45122 2007 5 PDF Available
Title
Use of sea water as salts source in starch- and soya bean-based media, for the production of Bacillus thuringiensis bioinsecticides
Abstract

In order to reduce the cost of Bacillus thuringiensis bioinsecticide-process production, a new medium only composed of starch, soya bean and diluted sea water, providing particularly 7.5 g/l NaCl, was optimized. The results obtained with cultures carried out into 1000 ml shake flasks showed 7% improvement of delta-endotoxin production in such cheap medium compared to that containing a mixture of pure minerals instead of sea water. Interestingly, this new formulated medium was shown to be efficient for delta-endotoxin production by several B. thuringiensis strains exhibiting various larvicidal activities towards either Diptera or Lepidoptera. Moreover, the use of such medium for large scale production of bioinsecticides was also evidenced in an automated full controlled 3 l fermenter. So that it was shown that sea water did not affect such requirements and have positive effect on growth, sporulation and delta-endotoxin synthesis. This should contribute to a significant reduction of the cost of B. thuringiensis bioinsecticide production and utilisation.

Keywords
Bacillus thuringiensis; Delta-endotoxin; Production; Media; Sea water; Starch
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Use of sea water as salts source in starch- and soya bean-based media, for the production of Bacillus thuringiensis bioinsecticides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 374–378
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us