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The elicited two-stage submerged cultivation of Antrodia cinnamomea for enhancing triterpenoids production and antitumor activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
3614 178 2012 7 PDF Available
Title
The elicited two-stage submerged cultivation of Antrodia cinnamomea for enhancing triterpenoids production and antitumor activity
Abstract

A two-stage submerged fermentation with elicitation was used to increase triterpenoids production of Antrodia cinnamomea in an airlift bioreactor with dual net draft tube. The dual net draft tube bioreactor had higher kLa value (7.3 h−1) than the solid tube bioreactor (5.5 h−1). The fungus was cultured at 25 °C for 12 days, then elicited with chemicals and physical factors. It was found that comparing to calcium chloride, chitosan was a more potent chemical elicitor. At dosage level of 100 mg/L, it not only resulted in more biomass production but also yielded higher crude triterpenoids production. The triterpenoids production could be significantly enhanced by means of the oxygen limitation and temperature fluctuation strategies. The maximum triterpenoids production could be achieved by using static culture (oxygen limitation) with temperature fluctuation between 25 °C (for 10 h) and 10 °C (for 14 h). The in vitro anticancer activity assay indicated that the crude triterpenoids produced by A. cinnamomea using the developed elicited two-stage cultivation protocol effectively inhibited proliferation of human cervical epithelioid carcinoma HeLa cells in a dosage dependent manner. The IC50 of the crude triterpenoids from the mycelium of A. cinnamomea after 21 days cultivation for HeLa cells was 25 μg/mL.

► Using the airlift reactor with dual net draft tube increased kLa and biomass content. ► Chitosan and CaCl2 as the chemical elicitation. ► Oxygen limitation and temperature fluctuation as physical elicitation. ► Elicitation by two-stage fermentation will increase triterpenoids content. ► The triterpenoids effectively inhibited proliferation of HeLa cells.

Keywords
Antrodia cinnamomea; Elicitation; Airlift bioreactors; Bioprocess design; Submerged culture; Viability
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The elicited two-stage submerged cultivation of Antrodia cinnamomea for enhancing triterpenoids production and antitumor activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 64, 15 May 2012, Pages 48–54
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us