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Immobilization of urease using glycidyl methacrylate grafted nylon-6-membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
36143 45122 2007 5 PDF Available
Title
Immobilization of urease using glycidyl methacrylate grafted nylon-6-membranes
Abstract

The graft copolymerization of glycidyl methacrylate (GMA) onto nylon-6-membrane using benzophenone (BP) as an initiator was carried out in an alcoholic aqueous solution. The acrylic double bond of GMA participated in the grafting onto the nylon-6-membrane backbone with the epoxy groups remaining unaffected. At the end of the grafting reaction, urease was immobilized onto the modified membrane. BP concentration, GMA concentration and organic solvent seperation were studied by determining the grafting percentage. The influence of urease concentration on the immobilization efficiency was also studied. With keeping other conditions constant, the optimum conditions were shown as following [BP]: 5 × 10−2 mM; [GMA]: 10 M; [urease]: 10 mg/ml, organic solvent: methanol.

Keywords
Urease immobilization; Nylon-6-membrane; Graft polymerization; Monomer; Glycidyl methacrylate; Benzophenone
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Immobilization of urease using glycidyl methacrylate grafted nylon-6-membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 439–443
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Online Support
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