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Optimization of the medium for glutathione production in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
36146 45122 2007 5 PDF Available
Title
Optimization of the medium for glutathione production in Saccharomyces cerevisiae
Abstract

As a powerful statistical experimental design, uniform design (UD) method has been successfully applied in various fields such as fermentation industry, pharmaceuticals, and others. In this paper, UD was applied to optimize the medium composition for glutathione production in shake-flask culture of Saccharomyces cerevisiae T65. The experiments of nine factors (glucose, yeast extract, peptone, malt extract, molasses, MgSO4, ZnSO4, (NH4)2HPO4 and thiamine) and nine levels were carried out according to the uniform design table U27(99). The experimental data was analyzed to obtain the regression model and the optimal medium composition was achieved by optimization with UD 3.0 software. The optimal medium consisted of 70 g/L glucose, 3 g/L yeast extract, 5 g/L peptone, 70 g/L malt extract, 20 g/L molasses, 5.6 g/L MgSO4, 16 mg/L ZnSO4, 7 g/L (NH4)2HPO4 and 0.2 mg/L thiamine. The GSH yield at the optimal point achieved 74.6 mg/L, which was 1.81 times higher than that of the control. The application of UD method resulted in enhancement in GSH production.

Keywords
Medium optimization; Uniform design; Glutathione production; Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 454–458
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
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