fulltext.study @t Gmail

One-step simultaneous purification of three water-soluble constituents in crude extracts from Danshen by adsorption chromatography on oligo-β-cyclodextrin substituted agarose gel media

Paper ID Volume ID Publish Year Pages File Format Full-Text
36151 45122 2007 6 PDF Available
Title
One-step simultaneous purification of three water-soluble constituents in crude extracts from Danshen by adsorption chromatography on oligo-β-cyclodextrin substituted agarose gel media
Abstract

A new adsorption chromatographic method for the simultaneous purification of Danshensu, Protocatechuic aldehyde and Salvianolic acid B (Lithospermic acid B) from a crude extract from radix of Danshen (Salviae miltiorrhiza) has been developed using oligo-β-cyclodextrin immobilized to Sepharose HP agarose gel media. Sample loads of up to 2.3 mg crude extracts/ml gel gave satisfactory results. The target components were eluted using step-wise isocratic elution with increasing concentrations of acetic acid. The separated fractions were identified by LC–MS. The recoveries of Danshensu, Protocatechuic aldehyde and Salvianolic acid B were 88.3%, 90.2% and 73.6% with purities of 99%, 99%, and 98%, respectively. Isocratic elution in 6% acetic acid gave baseline separation of Danshensu and Protocatechuic aldehyde, whereas 40% acetic acid was required for purification of Salvianolic acid B. Full separation efficiency could be restored by cleaning the column with 0.5 M NaOH followed by distilled water and 30% acetic acid.

Keywords
β-Cyclodextrin; Salviae miltiorrhizae; Danshensu; Protocatechuic aldehyde; Salvianolic acid B; Lithospermic acid B; Agarose gel media
First Page Preview
One-step simultaneous purification of three water-soluble constituents in crude extracts from Danshen by adsorption chromatography on oligo-β-cyclodextrin substituted agarose gel media
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 480–485
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us