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Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
36152 45122 2007 5 PDF Available
Title
Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli
Abstract

Three λ mutants were constructed based on the Q− mutant in order to enhance their productivity and stability in an Escherichia coli/bacteriophage λ system. The newly constructed bacteriophage mutants named λSNU1, λSNU2, and λSNU3 were Q−S−, Q−W−E−, and Q−S−W−E− mutants, respectively. Compared to all of the mutants, λSNU1 turned out to be the best with regards to higher protein expression and better genetic stability. Mechanisms by which these attributes are achieved have been discussed. The high productivity of P90c/λSNU1 for the recombinant protein was due to the high copy number of λ DNA and high translational efficiency. This mutant phage λSNU1 can be used to provide a high level of stability and productivity of the cloned gene particularly for long-term continuous operation.

Keywords
Plasmid stability; Bacteriophage λ; Q− Mutant; Late gene mutant; E. coli; Cloned gene expression
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Construction of various bacteriophage λ mutants for stable and efficient production of recombinant protein in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 42, Issue 3, March 2007, Pages 486–490
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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