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Optimal temperature control in fractional precipitation for paclitaxel pre-purification

Paper ID Volume ID Publish Year Pages File Format Full-Text
36159 45123 2006 5 PDF Available
Title
Optimal temperature control in fractional precipitation for paclitaxel pre-purification
Abstract

Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from plant cell cultures. The storage temperature of fractional precipitation was optimized in terms of the yield and purity of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v), and storage time (3 days). The greatest yield (∼84%) was obtained with storage at a constant temperature (0 °C) for 3 days. Conversely, the highest purity (∼79%) was obtained with stepwise reduction in temperature over 3 days. For a constant storage temperature, the maximum purity (∼67%) was obtained at 0 °C. There was little difference in the yield of paclitaxel between −20 and 12 °C. At a constant storage temperature (0 °C), both the precipitate yield and purity increased rapidly up to 18 h, and then increased more slowly. This pre-purification process serves to minimize solvent usage, and the size and complexity of the high-performance liquid chromatography (HPLC) operation required for paclitaxel purification.

Keywords
Paclitaxel; Fractional precipitation; Temperature; Pre-purification
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 2, February 2006, Pages 276–280
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us