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Beijerinckia indica L3 fermentation for the effective production of heteropolysaccharide-7 using the dairy byproduct whey as medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
36161 45123 2006 4 PDF Available
Title
Beijerinckia indica L3 fermentation for the effective production of heteropolysaccharide-7 using the dairy byproduct whey as medium
Abstract

A mutant strain of Beijerinckia indica L3 screened by a traditional chemical mutagenesis was grown in lactose-based mineral salts medium (MSM) for the production of heteropolysaccharide-7 (PS-7). The beta-galactosidase was expressed constitutively in the mutant and its activity was higher than that in parent strain. The highest amount of PS-7 produced by the mutant was 2.88 g/L with a viscosity of 4530 cP in lactose-based MSM medium. The PS-7 production was enhanced by the addition of 4 g/L glucose into lactose-based MSM medium, reaching 5.52 g/L with a viscosity of 39531 cP. PS-7 of 6.18 g/L with a viscosity of 45772 cP was produced from the mutant grown in whey medium. The PS-7 production from the mutant reached 7.04 g/L when 4 g/L glucose was added to the whey medium. Compositional analysis of PS-7 produced by the mutant grown in whey medium or lactose-based mineral salts medium showed that the ratios of glucose/rhamnose were maintained in the range similar to that of parent strain, indicating the same compositional structures of PS-7.

Keywords
Beijerinckia indica; Mutant; Heteropolysaccharide-7; Lactose; Whey; Beta-galactosidase
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Beijerinckia indica L3 fermentation for the effective production of heteropolysaccharide-7 using the dairy byproduct whey as medium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 2, February 2006, Pages 289–292
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us